Estimation of Salivary Levels of Pro Inflammatory Cytokines (Interleukin-1α and Interleukin-8) in Iraqi Lichen Planus patients, the association of these parameters with dental plaques, gingivitis and smoking status
DOI:
https://doi.org/10.32828/mdj.v13i1.821Keywords:
Oral Lichen Planus (OLP), Gingivitis, Cytokines (IL-1α, IL-8)Abstract
Background: Oral lichen planus (OLP) is chronic inflammatory disease of the oral mucosa whose etiology is still unknown but mounting evidence points to the immunologic basis of this disorder.
The aim of the present study was to assess the level of salivary IL-1α, IL- 8, salivary flow rate (SFR), the plaque and gingival indices in lichen planus patients, and to assess their level pre and post treatment with topical steroid in study group, in addition to the assessment of relation of smoking to these parameters in oral lichen planus patients.
Materials and methods: Whole saliva from fifteen (15) patients with oral lichen planus, eighteen (18) healthy persons, were investigated for the presence of IL-1 α, IL-8 by enzyme linked immunosorbent assay ( ELISA). In addition, the flow rate of saliva (SFR) measured in both groups, plaque and gingival indices also assessed pre and post treatment.
Results: The results showed a significant elevation of pro-inflammatory cytokines IL-1α , IL- 8, plaque and gingival indices in study group in comparison to control group except the SFR where it was higher in control group than study group also showed that some of these parameters significantly related to smoking statues.
The studies parameters also showed significant variation after using of topical steroid in which the salivary level of cytokines IL-1 α , IL-8, plaque &Gingival indices were significantly decreased after treatment except SFR was significantly increase after treatment.
Conclusion: Patients with oral lichen planus have had high levels of inflammatory cytokines in their saliva. The results also showed wide variations in levels among patients in pre and post treatment with steroid, in addition the level of pro inflammatory cytokines significantly related to smoking status. The results suggest links between the proinflammatory cytokines ( IL-1 α , IL-8 ) from one side and oral lichen planus disease, medication used for treatment and smoking status of the patients from another side.
References
- Al-Hashimi I, Schifter M, Lockhart PB, Wray D, Brennan M, Migliorati CA. Oral lichen planus and oral lichenoid lesions: diagnostic and therapeutic considerations. Radiol Endod 2007, 103, S25:1-12.
- Pezelj-Ribaric S, Prso IB, Abram M, Glazar I, Brumini G, et al. Salivary levels of tumor necrosis factor-alpha in oral lichen planus. Mediators Inflamm., 2004, 13: 131-133.
- Scully C, Eisen D, Carrozzo M. Management of oral lichen planus. Is J Clin Dermatol 2000, 1: 287-306.
- Vanja J. A. The Role of salivary cytokines in patients with oral lichen planus. Journal of Autacoids and Hormones, 2014, 3, 1:.2-6.
- Bouquot JE and Gundlach KK. Oral exophytic lesions in 23,616 white Americans over 35 years of age. Oral Surg Oral Med 1986: 62: 284–291.
- McCartan BE, Healy CM. The reported prevalence of oral lichen planus: a review and critique. J Oral Pathol Med 2008; 37:447–53.
- Lodi G, Scully C, Carrozzo M, Griffiths M, Sugerman PB, Thongprasom K. Current controversies in oral lichen planus: report of an international consensus meeting – Part 1. Viral infections and aetiopathogenesis. Oral Surg Oral Med, 2005,100, 40-51.
- Sugerman PB, Savage NW L.J. Walsh LJ, Zhao ZZ, Zhou XJ, Khan A, Seymour GJ and Bigby M. The Pathogenesis of oral lichen planus. Crit Rev Oral Biol Med 2002, 13:350-365.
- Erdem MT, Gulec AI, Kiziltunc A, Yildirim A, Atasoy M. Increased serum levels of tumor necrosis factor alpha in lichen planus. Dermatology 2003; 207: 367-70.
- Nicklin MJ, Weith A, Duff GW. A physical map of the region encompassing the human interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist genes. Genomics. 1994; 19 (2): 382–4.
- Bankers-Fulbright JL, Kalli KR, McKean DJ. Interleukin-1 signal transduction. Life Sci. 1996; 59 ,2: 61–83.
- Al-Ghurabi BH, Mohammed-Salih HS, Ghazi A, Saloom HF. Evaluation of salivary levels of proinflammatory cytokines (IL-1α, IL-8 and GM-CSF) in Adult orthodontic patients, IOSR-JDMS, 2014, 13, 3: 75-78.
- Gonzalez-Moles MA and Scully C. Vesiculoerosive oral mucosal disease – management with topical corticosteroids. fundamental principles and specific agents available. J Dent Res, 2005, 84, 294-301.
- Kłosek SK, Sporny S, Kanicka O.S, Kurnatowska A.J. Cigarette smoking induces overexpression of c-Met receptor in micro vessels of oral lichen planus. Arch Med Sci, 2011, 7, 4: 706-712.
- Silness J. and Loe H.: Correlation between oral hygiene and periodontal condition . Acta . Odont.Sc.1994,22:121-139 .
- Loe H: The gingival index, plaque index and retention index systems. J. periodontal, 1976 ,83:610 supplement.
- Lai Y, Shen Y, Liu XH, Zhang, Zeng Y, Liu YF. Interleukin-8 Induces the endothelial cell migration through the activation of phosphoinositide3-kinase-Rac1/RhoA pathway. Int J Biol Sci. 2011; 7: 782-791
- Sugawara S. Host defense of oral mucosa and the molecular mechanism of oral mucosal signal transduction diseases. J Oral Biosci. 2005, 47:115-125.
- Gornowicz A, Anna Byelaws 1, Zyta Grabowska SA, Wójcicka A. Zalewska.M, Maciorkowska.E, Pro-inflammatory cytokines in saliva of adolescents with dental caries disease, 2012; 19(4): 711-716.
- Pryor WA, Stone K: Oxidants in cigarette smokeRadicals,hydrogen peroxide, peroxynitrate, and peroxynitrite. Ann NYAcad Sci 1993, 686:12-27.
- Kode A, Yang S and Rahman I. Differential effects of cigarette smoke on oxidative stress and proinflammatory cytokine release in primary human airway epithelial cells and in a variety of transformed alveolar epithelial cells. 2006, 7:1:32.
- Gandolfo S, Richiardi L, Carrozo M. Risk of squamous cell carcinoma in 402 patients with oral lichen planus: a follow-up in an Italian population. Oral Oncol 2004, 40:77-83.
- Dietrich T, Reichart PA, Scheifele C. Clinical risk factors of oral leukoplakia in a representative sample of the US population. Oral Oncol 2004, 40: 158-63.
- Banoczy J, Gintner Z, Dombi C. Tobacco use and oral leukoplakia. J Dent Educ 2001, 65: 322-7.
- Newman MG, Takei HH, Klokkevold PR, Carranza FA. Carranza’s F. Clinical Periodontology. 10th ed. Saunders Elsevier: Missouri 2007; 254-5
- Kedah S, Rashed L, Obaia E, Sabry D. A preliminary study: matrix metalloproteinase expression as an indicator of the hazards of shisha (nargila) smoking. Arch Med Sci 2009; 5: 570-6.
- Lodi G, Scully C, Carrozzo M, Griffiths M,Sugerman PB, Thongprasom K . Current controversies in oral lichen planus; report of an international consensus meeting. Part 2. Clinical management and malignant transformation. Oral Surg Oral Med Oral Pathol Oral Radiol Endod ,2005,100,164-178.
- Thompson K and Dhanuthai K. Steriods in the treatment of lichen planus: a review. Journal of Oral Science,2008, Vol. 50, No. 4, 377-385.
- Fluixa CRl, StatSebastain JB,Masanet MM. and Scally C Peridontal status in patients with oral lichen planus : a study of 90 cases .Oral Disease2008,5,4:303-306.
- Ling J,Fan M W,Wang SZ,Chen X M .A clinical study of 674 patients with oral lichen planus in chine .Journal of Oral Pathology and Medicine ,2005,34, 8: 467-472
- Ali AM , Elgendy E A , Elden A. A comparative Study between Intralesional low molecular weight chitosan and triamcinolone acetonide for treatment of erosive –atrophic oral lichen planus. J. of American Science ,2011,7,10 :338-345.
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This work is licensed under a Creative Commons Attribution 4.0 International License.
