Relation between salivary Matrix-metalloproteinase-8 with periodontal health; dental biofilm induced gingivitis, localized and generalized periodontitis
DOI:
https://doi.org/10.32828/mdj.v19i1.991Keywords:
Gingivitis, Matrix metallo-proteinase-8, Periodontitis, SalivaAbstract
Aim of the study: was to find the potential relation between the salivary concentration of matrix metalloproteinase-8 with periodontal health and disease by examining its’ level in individuals with healthy periodontium, with dental biofilm induced gingivitis and with periodontitis (localized and generalized).
Material and method: Saliva was collected from 90 participants with an age range from (20-60) years, all of them were systemically healthy. 25 cases were enrolled under the dental Biofilm induced gingivitis group, 25 cases under the localized periodontitis group, and 25 cases under the generalized periodontitis group, with 15 subjects enrolled in the healthy periodontium(control) group. Detection of MMP-8 in saliva was achieved by utilizing Enzyme-Linked Immunosorbent Assay (ELISA).
Results: The present data showed that MMP-8 was higher significantly(P<0.05) in disease groups (dental Biofilm induced gingivitis, localized and generalized periodontitis groups) as compared to the healthy (control) group. With a significant difference between dental biofilm-induced gingivitis and generalized periodontitis groups.
Conclusion: There is a positive relationship between salivary matrix metalloproteinase-8 with periodontal health and disease.
References
HUANG, N. & GIBSON, F. C. 2014. Immuno-pathogenesis of periodontal disease: current and emerging paradigms. Curr oral health Rep, 1, 124-132.
CHAPPLE, I. L., MEALEY, B. L., VAN DYKE, T. E., BARTOLD, P. M., DOMMISCH, H., EICKHOLZ, P., GEISINGER, M. L., GENCO, R. J., GLOGAUER, M. & GOLDSTEIN, M. 2018. Periodontal health and gingival diseases and conditions on an intact and a reduced periodontium: Consensus report of workgroup 1 of the 2017 World Workshop on the Classification of Periodontal and Peri‐Implant Diseases and Conditions. J periodontol, 89, S74-S84.
TONETTI, M. S., GREENWELL, H. & KORNMAN, K. S. 2018. Staging and grading of periodontitis: Framework and proposal of a new classification and case definition. J periodontol, 89, S159-S172.
MILWARD, M. R. & ROBERTS, A. 2019. Assessing periodontal health and the british society of periodontology implementation of the new classification of periodontal diseases 2017. Dent Update, 46, 918-929.
TABA, M., KINNEY, J., KIM, A. S. & GIANNOBILE, W. V. 2005. Diagnostic biomarkers for oral and periodontal diseases. Dental Clinics, 49, 551-571.
GROUP, B. D. W., ATKINSON JR, A. J., COLBURN, W. A., DEGRUTTOLA, V. G., DEMETS, D. L., DOWNING, G. J., HOTH, D. F., OATES, J. A., PECK, C. C. & SCHOOLEY, R. T. 2001. Biomarkers and surrogate endpoints: preferred definitions and conceptual framework. Clin Pharmacol ther, 69, 89-95.
FUENTES-ARDERIU, X. 2013. What is a biomarker? It’s time for a renewed definition. Clin Chem Lab Med, 51, 1689-1690.
ZHANG, L., LI, X., YAN, H. & HUANG, L. 2018. Salivary matrix metalloproteinase (MMP)-8 as a biomarker for periodontitis: A PRISMA-compliant systematic review and meta-analysis. Medicine, 97.
FRANCO, C., PATRICIA, H.-R., TIMO, S., CLAUDIA, B. & MARCELA, H. 2017. Matrix metalloproteinases as regulators of periodontal inflammation. Int J Mol Sci, 18, 440.
OWEN, C. A., HU, Z., LOPEZ-OTIN, C. & SHAPIRO, S. D. 2004. Membrane-bound matrix metalloproteinase-8 on activated polymorphonuclear cells is a potent, tissue inhibitor of metalloproteinase-resistant collagenase and serpinase. The Journal of Immunology, 172, 7791-7803.
KIILI, M., COX, S., CHEN, H., WAHLGREN, J., MAISI, P., ELEY, B., SALO, T. & SORSA, T. 2002. Collagenase‐2 (MMP‐8) and collagenase‐3 (MMP‐13) in adult periodontitis: molecular forms and levels in gingival crevicular fluid and immunolocalisation in gingival tissue. J Clin Periodontol, 29, 224-232.
ZIEBOLZ, D., SCHMALZ, G., GOLLASCH, D., EICKHOLZ, P. & RINKE, S. 2017. Microbiological and aMMP-8 findings depending on peri-implant disease in patients undergoing supportive implant therapy. Diagn Microbiol Infect Dis, 88, 47-52.
SEXTON, W. M., LIN, Y., KRYSCIO, R. J., DAWSON III, D. R., EBERSOLE, J. L. & MILLER, C. S. 2011. Salivary biomarkers of periodontal disease in response to treatment. J clin Periodontol, 38, 434-441.
YAKOB, M., MEURMAN, J., SORSA, T. & SÖDER, B. 2013. Treponema denticola associates with increased levels of MMP‐8 and MMP‐9 in gingival crevicular fluid. Oral Dis, 19, 694-701.
SYNDERGAARD, B., AL‐SABBAGH, M., KRYSCIO, R. J., XI, J., DING, X., EBERSOLE, J. L. & MILLER, C. S. 2014. Salivary biomarkers associated with gingivitis and response to therapy. J Periodontol, 85, e295-e303.
SORSA, T., GURSOY, U. K., NWHATOR, S., HERNANDEZ, M., TERVAHARTIALA, T., LEPPILAHTI, J., GURSOY, M., KÖNÖNEN, E., EMINGIL, G. & PUSSINEN, P. J. 2016. Analysis of matrix metalloproteinases, especially MMP‐8, in gingival crevicular fluid, mouthrinse and saliva for monitoring periodontal diseases. Periodontol 2000, 70, 142-163.
ZHANG, C. Z., CHENG, X. Q., LI, J. Y., ZHANG, P., YI, P., XU, X. & ZHOU, X. D. 2016. Saliva in the diagnosis of diseases. Int J Oral Sci, 8, 133-7.
AHMED, R. F. & AL-JUBOURI, R. H. 2017. Oral Health Status, Salivary MMP-8& Secretory Leukocyte Peptidase Inhibitor (SLPI) Among Uncontrolled Type-I Diabetes Mellitus In Iraqi Patients. J. Baghdad coll. Dent, 29, 65-70.
MALAMUD, D. 2011. Saliva as a diagnostic fluid. Dental Clinics, 55, 159-178.
BUCZKO, P., ZALEWSKA, A. & SZARMACH, I. 2015. Saliva and oxidative stress in oral cavity and in some systemic disorders. Journal of physiology and pharmacology: an official journal of the Polish Physiological Society, 66, 3-9.
O'Leary, T.J., Drake, R.B. and Naylor, J.E., 1972. The plaque control record. J Periodontol, 43(1), pp.38-38.
AINAMO, J. & BAY, I. J. I. D. J. 1975. Problems and proposals for recording gingivitis and plaque. 25, 229-235.
DYE, B. A. & SELWITZ, R. H. J. J. O. C. P. 2005. The relationship between selected measures of periodontal status and demographic and behavioural risk factors. 32, 798-808.
LISTGARTEN, M. J. J. O. C. P. 1980. Periodontal probing: what does it mean? 7, 165-176.
NAVAZESH, M. 1993. Methods for collecting saliva. Ann N Y Acad Sci, 694, 72-77.
ALJEHANI, Y. A. 2014. Risk factors of periodontal disease: a review of the literature. Int J Dent, 2014.
MOHAMMED, H. A., ABDULKAREEM, A. A., ZARDAWI, F. M. & GUL, S. S. 2022. Determination of the Accuracy of Salivary Biomarkers for Periodontal Diagnosis. Diagnostics, 12, 2485.
ALDHAHER, Z. A., AL-GHURABI, B. H. & ALWAN, B. H. 2018. Serum Levels of IL-22 and ACPA in Patients with Rheumatoid Arthritis. Journal of PurE and aPPliEd Microbiology, 12, 687-691.
BOYAPATI, R., CHERUKURI, S. A., BODDURU, R. & KIRANMAYE, A. 2021. Influence of Female Sex Hormones in Different Stages of Women on Periodontium. J Midlife Health, 12, 263- 266.
AL-KARAWI, S. E. & AL-RUBAIE, M. S. 2014. Estimation of soluble CD14 level in saliva of patients with different periodontal conditions and its correlation with periodontal health status. J. Baghdad Coll. Dent, 26, 138-143.
SALMAN, S. A. 2015. Measurement of salivary Immunoglobulin A of participants with a healthy, gingivitis and chronic periodontitis conditions. J. Baghdad Coll. Dent, 27, 120-123.
MOUSA, H. A. & SALIEM, S. S. 2016. Assessment of Alkaline Phosphatase, Salivary Flow Rate and Salivary Potential of Hydrogen in Relation to Severity of Chronic Periodontitis. J. Baghdad Coll. Dent, 28, 126-131.
STROHM, T. F. & ALT, K. W. 1998. Periodontal disease—etiology, classification and diagnosis. Dental anthropology. Springer.
RAI, B., KHARB, S., JAIN, R. & ANAND, S. C. 2008. Biomarkers of periodontitis in oral fluids. J Oral Sci, 50, 53-56.
RANGBULLA, V., NIROLA, A., GUPTA, M., BATRA, P. & GUPTA, M. 2017. Salivary IgA, interleukin-1β and MMP-8 as salivary biomarkers in chronic periodontitis patients. Chin J Dent Res, 20, 43-51.
Carranza, F.A. 2009. Clinical Periodontology, Philadelphia, WB Sounder Company,10th Edition.
GUPTA, N., GUPTA, N., GUPTA, A., KHAN, S. & BANSAL, N. 2015. Role of salivary matrix metalloproteinase-8 (MMP-8) in chronic periodontitis diagnosis. Front Med, 9, 72-76.
NEWMAN, M., TAKEI, H. & KLOKKEVOLD, P. 2012. Carranza’s Clinical Periodontology 11th edition St. Louis: Mosby, 414-418.
ROMERO-CASTRO, N. S., VÁZQUEZ-VILLAMAR, M., MUÑOZ-VALLE, J. F., REYES-FERNÁNDEZ, S., SERNA-RADILLA, V. O., GARCÍA-ARELLANO, S. & CASTRO-ALARCÓN, N. 2020. Relationship between TNF-α, MMP-8, and MMP-9 levels in gingival crevicular fluid and the subgingival microbiota in periodontal disease. Odontology, 108, 25-33.
PAGE, R. C. & KORNMAN, K. S. 1997. The pathogenesis of human periodontitis: an introduction. Periodontol 2000, 14, 9-11.
SORSA, T., TJÄDERHANE, L., KONTTINEN, Y. T., LAUHIO, A., SALO, T., LEE, H. M., GOLUB, L. M., BROWN, D. L. & MÄNTYLÄ, P. 2006. Matrix metalloproteinases: contribution to pathogenesis, diagnosis and treatment of periodontal inflammation. Annals of medicine, 38, 306-321.
HERR, A. E., HATCH, A. V., THROCKMORTON, D. J., TRAN, H. M., BRENNAN, J. S., GIANNOBILE, W. V. & SINGH, A. K. 2007. Microfluidic immunoassays as rapid saliva-based clinical diagnostics. Proceedings of the National Academy of Sciences, 104, 5268-5273.
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This work is licensed under a Creative Commons Attribution 4.0 International License.
