The antibacterial effect of Ellagic acid on Lacto bacillus acidophilus in comparison to Chlorhexidin
DOI:
https://doi.org/10.32828/mdj.v12i1.837Keywords:
Ellagic acid, LactobacillusAbstract
Ellagic acid is a natural herb extract were prepared from flesh pomegranate extract. The preparation and experiments were done and conducted in a laboratory of chemistry department of Ministry of Science and Technology. Biological activity of Ellagic acid to ward pathogenic bacteria (Lactobacillus acidophilus) was studied. The purpose of this study was to evaluate the antibacterial effect of prepared Ellagic acid with different concentrations on growth of lacto bacillus acidophilus in comparison with Chlorhexidine gluconate 0.2% and deionized water. This in vitro bacteriological study was tested according to agar well diffusion technique. The results showed that a highly significant increase in mean values of Ellagic acid compared to Chlorhexidine and deionized water. The concentration, 5 mg/ml and10 mg/ml of Ellagic acid showed stronger effects in inhibition of lactobacillus acidophilus, than Chlorhexidine 0.2%. Ellagic acid 5and10mg/ml has the ability to interfere with the adherence of Lactobacilli in vitro.
Previous study showed that Ellagic acid had antibacterial effect on Streptococcus mutans , Ellagic acid could be used as antibacterial mouth washes to prevent dental caries.
Chlorhexidine.
References
- Marsh,P.D."Microbiological aspects of the Chemical control aspects of the chemical control of plaque and gingivitis.J.DentalRes.1992; 71:1431-1438.
- Hardie JM,." The microbiology of dental caries" 1982. Dent Update 1982; 9 (4): 199-200,202-4,206-8.PMD6959931.
- Rogers AH (editor) "Molecular oral Microbiology, 2008.Caister, Academic press. ISBN, 978-1-9044 55-24-0.
- Featherston J.D. Prevention and reversal of dental caries, role of low level fluoride. Community Dental Epidemiol 1999;27:31-40
- Klinberg,, I; A mixed-bacteria ecological approach to understanding the role of oral bacteria in dental caries causation: an alternative to Streptococus mutans and specific plaque hypothesis. Critical Reviews in oral Biology &Medicine, 2002; 13(2):108-125.
- Douglass,JM;LI,Y; Tinanoff,N."Association ofmutans streptococci between caregivers and their children"Pediatric Dentistry 2008 .30(5):375-87.
- Curran, H.R..,Rogers,L.A.,Whittier,E.O.;The distinguishing characteristics of Lactobacillus Acidophillus,2006; JoutrnalBacteriolog , 25(6):595-621;1933.
- Altemann, Eric, etal, 2005 "Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus NCFMPNAS Vol: 1`02, No.11. http//www.panas org/ content/102/11/3906.full.pdfthtml.
- HolzapfelWH.HabererP.GeisenR,etal; Taxonomy and important features of probiotic microorganisims in food and nutrition .AmJ.Clin. .N utr.2001;73(2) :365s-373s.
- 8abOral Health Topics: Cleaning your teeth and gum. Hosted on the American Dental Association Website. Page accessed August 15, 2006.
- FShahidi and Minacs. Phenolic in food and nutraceuticals .CRC press, USA, 2003, PP.85.
- SanakaS,AizawaM,Kim M, Yammamoto.T. inhibilitory effects of polyphenols on growth and cellular adherence of an oral bacterium prophyromonasgingivivalis. BiotechnolBiochem .1996;745-746.
- Lesso JN,Bansode RR, Trappy, BawadiHA and TruaxR.Invitroantiproliferative activities of ellagic acids NutrBiochem .2004; 15 (11):672-678.
- Muslim. S. The antibacterial effects OF Ellagic acid on Mutansstreptoocci in comparison to chlorhexidine(invitro and invivo study), A thesis submitted to the collage of Dentistry Baghdad University, 2009.
- Fukamoto Y, Ashik and Yama Zaki, :Methol for producing ellagic acid, Jap. Patent, NO. 2002-25003.
- Prakash, S.etal."The Gut Microbiota and Human Health with Emphasis on the Use of Microen capsulated Bacterial cells" Journal of Biomedicine and Biotechnology(2011)..
- G.A.Washington. Laboratory procedures in clinical bmicrobiology Spring Verlog. NewYork.1981.
- EL-Samarrai S.Major and trace elements contents of permanent teeth and saliva, among a group of adolescents, in relation to dental caries,gingivitis and mutans streptococci (invivo and invitro study) .PhD. Thesis, College of dentistry, University of Baghdad, Iraq,2001.
- Hwang JM, Cho JS,KimTH,LeeYl"Ellagic acid prptects hepatocytes fom damage by inhibiting mitochondrial production of reactive oxygen species. Biomed. Phamacother .{Epub ahead of print} ,2009.
- Scalbert A. Antibacterial properties of tannins. Chemistry 1999, 1, 30: 3875-3883.
- Grag.W, and Gldine .M. Bioavailability, and bioafficay of polyphenols in human, AmJClinNutr.. 2005, 81(1)243-255.,
- Menaker,L. "The biologic basis of dental caries an oral text book, Ed. Harper and Raw.Newyork-1980.pp.210-255,207-312
- ThylstrupA.and.FejersTextbook of Clinical Cariology.2nded. Munksgaard.Copenhagen.1996:11-158.
Downloads
Published
How to Cite
Issue
Section
Categories
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
