Cytotoxic Effect of Nano Polyvinylidene Fluoride On Human Gingival Fibroblast
DOI:
https://doi.org/10.32828/mdj.v21i1.1180Keywords:
Nano PVDF, Cytotoxicity, MTT-Assay, Sodium fluoride varnish, Human gingival fibroblast, BiocompatibilityAbstract
Aim: This investigation is the first to investigate the cytotoxic effects of Nano Polyvinylidene-Fluoride (PVDF) on Human Gingival Fibroblasts (HGF) cells in comparison to sodium fluoride (NaF) varnish .The objective of the investigation is to assess the potential of Nano-PVDF as a safer and potential alternative to fluoride-based treatments by comparing the cytotoxicity of three different PVDF concentrations ( 1.26%, 2.26%, and 3.26%) to NaF varnish (2.26%). Method: The MTT-Assay was performed to evaluate the cytotoxicity of the tested materials on HGFs. To assess the toxic effects ,cell viability after the application of NaF varnish (2.26%) and PVDF (1.26%, 2.26%, and 3.26%) at each concentration.Results:The finding indicated that all concentrations of PVDF exhibited lower cytotoxicity compared to NaF varnish. Within the PVDF groups , the 3.26% concentration demonstrated the least cytotoxicity, succeeded by 2.26% and 1.26% respectively. On the other hand, NaF varnish exhibited the greatest toxicity among the evaluated substances.Conclusion:This innovative study demonstrates that Nano-PVDF, particularly at a concentration of 3.26%, is a promising material exhibiting enhanced biocompatibility and less cytotoxicity relative to traditional NaF varnish. All data represented that Nano-PVDF may function as a safer option in dental treatments, with exhibiting little adverse effects on oral cells.
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