The effect of ozone gel as adjunct treatment in patients of periodontitis according to bleeding on probing and platelet-activating factor (A split-mouth randomized controlled clinical trial)
DOI:
https://doi.org/10.32828/mdj.v19i1.993Keywords:
ozone gel, bleeding on probing, platelet-activating factorAbstract
Materials and methods: total sample composed of 58 periodontal pockets was divided into two groups. The control group had 28 periodontal pockets treated with scaling and root planning only, while the test group had 30 periodontal pockets treated with scaling and root planning and ozone gel. Two applications of the gel were applied at baseline and one month after the treatment .platelet activating factor (biomarker) and clinical parameters of bleeding on probing are measured at baseline, after one month, and after three months. In the second visit of periodontal therapy (after one day), GCF samples were obtained from patients by gently inserting periocol paper into the selected depth of the pocket when resistance was encountered. The periocol paper was then left in position for 30 seconds before being removed and weighed on a chemical balance. Each paper strip was placed in a tube keeping o.3ml of buffer phosphate saline, then transferred and stored at -40C. Calculated the difference between the weight of periocol paper before and after exudate absorption of gingival crevicular fluid. After sample storage for five minutes, the samples were centrifuged at 13,000 rpm.
Results: Test sites showed a greater reduction in bleeding on probing and there was a significant difference in the first month only when compared to control sites (Fisher exact=0.010) but no significant difference at three months. The platelet-activating factor was reduced significantly by ozone gel in the first month with only a p-value (0.000) but no significance at one and three months.
Conclusion: In comparison to SRP alone, treatment with ozone gel as an adjuvant therapy yields no statistically significant improvement after three months.
References
ALJUBOORI, Ismael W.; MAHMOOD, Maha Sh. The Effects of Salvia officinalis Gel as an Adjunct to Scaling and Root Planning in Patients with Periodontitis (Clinical and Immunological Study). Int. J. Drug Deliv. Technol, 2020, 10: 232-237.
ARMITAGE, Gary C. Analysis of gingival crevice fluid and risk of progression of periodontitis. Periodontology 2000, 2004, 34.1: 109-119.
LAMSTER, Ira B.; AHLO, Joseph K. Analysis of gingival crevicular fluid as applied to the diagnosis of oral and systemic diseases. Ann N Y Acad Sci, 2007, 1098.1: 216-229.
MOHAMMED-SALIH, Harraa S.; SALOOM, Hayder F. Collection, storage and protein extraction method of gingival crevicular fluid for proteomic analysis. Baghdad Sci. J, 2021, 19: 368-377.
GUENTSCH, Arndt, et al. Comparison of gingival crevicular fluid sampling methods in patients with severe chronic periodontitis. J periodontol, 2011, 82.7: 1051-1060.
FADHIL, Raghad. The association of crevicular albumin level with the severity of periodontal destruction in chronic periodontitis patients after initial periodontal treatment. J Baghdad Coll Dent, 2014, 26.1: 134-137.
ZHONG, Y., et al. Gingival crevicular fluid interleukin‐1β, prostaglandin E2 and periodontal status in a community population. J clin periodontol, 2007, 34.4: 285-293.
BOSTANCI, Nagihan, et al. Gingival crevicular fluid levels of RANKL and OPG in periodontal diseases: implications of their relative ratio. J clin periodontol, 2007, 34.5: 370-376.
PRESCOTT, Stephen M., et al. Platelet-activating factor and related lipid mediators. Annu Rev Biochem, 2000, 69.1: 419-445.
STAFFORINI, Diana M., et al. Platelet-activating factor, a pleiotrophic mediator of physiological and pathological processes. Crit Rev Clin Lab Sci, 2003, 40.6: 643-672.
CHEN, Hui, et al. Platelet-activating factor levels of serum and gingival crevicular fluid in nonsmoking patients with periodontitis and/or coronary heart disease. Clin oral investig 2010, 14: 629-636.
AHMMAD, Ban Z.; IBRAHEEM, Leqaa M. Anti-Microbial Effect Of Different Times exposure of ozonized Gas And Ozonized Water On periodontal pathogens (In Vitro Study). J Baghdad Coll Dent, 2017, 29.2: 78-82.
AKDENIZ, Sıdıka Sinem, et al. The effects of ozone application on genotoxic damage and wound healing in bisphosphonate-applied human gingival fibroblast cells. . Clin oral investig, 2018, 22: 867-873.
SHOUKHEBA, M. Y. M.; ALI, Sh A. The effects of subgingival application of ozonated olive oil gel in patient with localized aggressive periodontitis. A clinical and bacteriological study. Tanta Dental Journal, 2014, 11.1: 63-73.
CATON, Jack G., et al. A new classification scheme for periodontal and peri‐implant diseases and conditions–Introduction and key changes from the 1999 classification. J Periodontol, 2018, 89: S1-S8.
NEWMAN, Michael G., et al. Carranza's clinical periodontology. Elsevier health sciences, 2011.
KALLIO, Elisa, et al. Lipopolysaccharide: a link between periodontitis and cardiometabolic disorders. 2014.
COLOMBO, Marco, et al. Ozone gel in chronic periodontal disease: A randomized clinical trial on the anti-inflammatory effects of ozone application. Biology, 2021, 10.7: 625.
KSHITISH, Durga; LAXMAN, Vandana K. The use of ozonated water and 0.2% chlorhexidine in the treatment of periodontitis patients: A clinical and microbiologic study. Indian J Dent Res, 2010, 21.3: 341.
MI, Ramzy, et al. Management of aggressive periodontitis using ozonized water. Egypt Med JNR C, 2005, 6.1: 229-45.
SKURSKA, A., et al. Evaluation of the influence of ozonotherapy on the clinical parameters and MMP levels in patients with chronic and aggressive periodontitis. Adv Med Sci, 2010, 55.2: 297-307.
HAYAKUMO, Sae, et al. Clinical and microbiological effects of ozone nano-bubble water irrigation as an adjunct to mechanical subgingival debridement in periodontitis patients in a randomized controlled trial. Clin Oral Investig, 2013, 17: 379-388.
GRAY, Nicholas F. Ozone disinfection. In: Microbiology of waterborne diseases. Academic press, 2014. 599-615.
AL HABASHNEH, R.; ALSALMAN, W.; KHADER, Y. Ozone as an adjunct to conventional nonsurgical therapy in chronic periodontitis: a randomized controlled clinical trial. J periodontal Res, 2015, 50.1: 37-43.
TASDEMIR, Zekeriya, et al. The effects of ozone therapy on periodontal therapy: A randomized placebo‐controlled clinical trial. Oral diseases, 2019, 25.4: 1195-1202.
TUNKEL, Jochen; HEINECKE, Achim; FLEMMIG, Thomas Frank. A systematic review of efficacy of machine‐driven and manual subgingival debridement in the treatment of chronic periodontitis. J clinl Periodontol, 2002, 29: 72-81.
KELES, G. C., et al. Levels of platelet activating factor in gingival crevice fluid following periodontal surgical therapy. . J periodontal Res, 2006, 41.6: 513-518.
ISHII, Satoshi; SHIMIZU, Takao. Platelet-activating factor (PAF) receptor and genetically engineered PAF receptor mutant mice. Progress in lipid research, 2000, 39.1: 41-82.
ISHIHARA, Yuichi, et al. Regulation of immunoglobulin G2 production by prostaglandin E2 and platelet-activating factor. Infection and immunity, 2000, 68.3: 1563-1568.
STAFFORINI, Diana M., et al. Platelet-activating factor, a pleiotrophic mediator of physiological and pathological processes. Crit Rev Clin Lab Sci, 2003, 40.6: 643-672.
SCHENKEIN, Harvey A., et al. Mechanisms underlying the association between periodontitis and atherosclerotic disease. Periodontology 2000, 2020, 83.1: 90-106.
SCHMIDMAIER, Gerhard, et al. Biodegradable polylactide membranes for bone defect coverage: biocompatibility testing, radiological and histological evaluation in a sheep model. Clin Oral Implants Res, 2006, 17.4: 439-444.
SUBBARAO, Krishnan Chandragiri, et al. Gingival crevicular fluid: an overview.
J Pharm Bioallied Sci, 2019, 11.Suppl 2: S135.
EASTON, Alexander S. Regulation of permeability across the blood-brain barrier. Biology and Regulation of Blood-Tissue Barriers, 2013, 1-19.
URAZ, Ahu, et al. Ozone application as adjunctive therapy in chronic periodontitis: Clinical, microbiological and biochemical aspects. Journal of dental sciences, 2019, 14.1: 27-37.
OH, Hyun, et al. Effects of initial periodontal therapy on interleukin-1β level in gingival crevicular fluid and clinical periodontal parameters. J oral sci, 2015, 57.2: 67-71.
QADRI, Talat; TUNÉR, Jan; GUSTAFSSON, Anders. Significance of scaling and root planing with and without adjunctive use of a water-cooled pulsed Nd: YAG laser for the treatment of periodontal inflammation. Lasers Med Sci, 2015, 30: 797-800.
NAGARAJAN, Radha, et al. Cross‐talk between clinical and host‐response parameters of periodontitis in smokers. J periodontal Res, 2017, 52.3: 342-352.
CHEN, T. L., et al. Effects and holding time of hyperbaric oxygen on human severe periodontitis. Shanghai Kou Qiang Yi Xue , 2003, 12.6: 403-405.
NAKAMURA, Hideyasu, et al. Reviewing the 20 years of operation of ozonation facilities in Hanshin Water Supply Authority with respect to water quality improvements. Ozone: Science & Engineering, 2017, 39.6: 397-406.
EMINGIL, Gülnur, et al. Levels of platelet‐activating factor in gingival crevicular fluid and gingival tissue in specific periodontal diseases. J periodontol, 2001, 72.8: 1032-1037.
GÜNDAY, Sevim, et al. Analysis of daytime variations in gingival crevicular fluid: a circadian periodicity?. J Periodontol, 2014, 85.3: e47-e56.
ZHENG, Pei, et al. Periodontal parameters and platelet‐activating factor levels in serum and gingival crevicular fluid in a Chinese population. J clin Periodontol, 2006, 33.11: 797-802.
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