Identification of mature and immature (osteoid production) in ossifying fibroma by Tetrachromic verdeluz orange G-acid fuchsin (VOF) stain versus hematoxylin and eosin stain
DOI:
https://doi.org/10.32828/mdj.v18i2.927Keywords:
ossifying fibroma, osteoid, hematoxylin and eosin stain, VOFstainAbstract
Aim of Study: Identification of mature and immature (osteoid production) in ossifying fibroma using histochemical study by hematoxylin and eosin (H&E) and tetrachromic verdeluz orange G-acid fuchsin (VOF) stains.
Materials and Methods: Forty five tissue sections divided into three groups made up the study's data. Twenty portions from the archives, which made up Group 1, exhibited ossifying fibroma, group 2 comprised fifteen psammatoid juvenile ossifying fibroma sections and group 3 comprised ten section of trabecular juvenile ossifying fibroma. Each block was divided into two 5 m sections, with one section stained with hematoxylin and eosin (H & E) and the other with the tetrachromic Verde Luz-orange G-acid fuchsin (VOF). Histomorphometric examination of tissues is based on quantitative measurements of microscopic osteoid production and has been used to provide information on tissue pathology in these lesions
Results: The results showed female predominance in OF cases comprising (70.0%).Whereas male predominance found in psammatoid and trabecular juvenile ossifying fibroma comprising (60.0%), (70.0%) respectively .Ossifying fibroma and juvenile ossifying fibroma were distributed between the maxilla and mandible in the following ways: (65.0%) of OF were in the mandible, (73.3%) of PsJOF were in the maxilla, and (60.0%) of TrJOF were in the mandible. By using ANOVA test however the result non-significant OF show high mean value OF osteoid followed by PsJOF then TrJOF in conventional H&E while in VOF stains PsJOF showed high mean value followed by OF and finally TrJOF. Using paired t-test H&E showed a high mean values of osteoid in OF, TrJOF and PsJOF, compared to VOF stain, however statistically non-significant compared mean value of osteoid between H&E stain and VOF in TrJOF there was a border line significant (0.055).
Conclusion: Our awareness of these lesions and our ability to distinguish and identify them is improved by a study of the histological characteristics of ossifying fibroma and its subtype. H&E staining necessitates numerous steps, whereas VOF only needs one step.
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