Evaluation the effect of different instrumentation and obturation techniques on apical microleakage . (In vitro comparative study)

Abstract

This in vitro study evaluated and compared the apical sealing ability of two
obturation techniques and two type of instrumentation. A total of 120 teeth were
collected randomly divided into two groups 60 roots prepared using hand protaper,
other 60 roots prepared using rotary protaper .Each group was subdivided randomly
into two obturation groups of 30 roots for each; in group A- obtura II was used ,and
in group B-thermafill (TF) was used. Then the samples in all groups left for a week at
room temperature. After coronal sealing, all surfaces of the root covered with three
layer of nail polish except the apical 2mm, then each sample immersed in 10% black
Indian ink dye for 72 hours .After that the samples rinsed and nail polish removed
.then samples cleared & decalcified.Data had been collected from independent
examiners and statistically analyzed using student t-test. The stereomicroscopic
results showed that there was highly significant difference between hand and rotary
instrumentation, with in favor of rotary instrumentation technique. Concerning the
obturation techniques, Thermafill showed best apical sealing followed by Obtura II;
however, the differences were not significant.