Antibacterial activity of Silver Nanoparticles Using Stem bark of Juglans regia against, Streptococcus mutans , Streptococcus. sanguis and ,Porphyromonas gingivalis

Abstract

Dental careis & periodontal disease ,the most widespread diseases affecting
mankind ,involve the adherence of bacteria & development of biofilms on both the
natural & restored tooth surface .within this context, a biofilm can be Classed as an
aggregate of micro-organisms in which cell adhere to each other & to a surface.
Nanotherapeutics offers the possibilitiy to control the formation of these & other oral
biofilms through the use of nanoparticles with biocidal ,anti-adhesive & delivery
capabilities.
In this study, we investigated the antimicrobial activity of silver nanoparticles
(Ag-NPs) using medicinal plant extracts (Stem bark of Juglans regia ) against,
Streptococcus mutans, Streptococcus. Sanguis, and Porphyromonas gingivalis.
Extracellular biosynthesis of silver nanoparticles was carried out by using
medicinal plant extracts for the reduction of aqueous silver ions in short period. The
silver nanoparticles formation was confirmed by the color change of plant extracts
(SNPs) and further confirmed with the help of UV-Vis spectroscopy. Antimicrobial
effect of Ag-NPs against, Strep.mutans, Strep. sanguis , and Porph. gingivalis was
investigated by using disc diffusion method.
The growth of Gram-positive Strep.mutans, Strept. sanguis (the causative agents
of careis) , and Porph. Gingivalis (the causative agents of Periodontitis) were
inhibited by Ag-NPs. The highest antimicrobial activity was observed against Strep.
mutans (15.6mm SD± 1.6), Strept. sanguis (13.3mm SD±1.4) and the least was
noticed against Porph. gingivalis (12mm SD± 1.0).
It is confirmed that silver nanoparticles are capable of rendering high antibacterial
efficacy and hence has a great potential in the preparation of drugs used against oral
bacterial diseases.