An evaluation of the Antimicrobial Activity of Five Endodontic Sealers on three bacterial species. (In vitro study)

Abstract

To evaluate the antimicrobial action of five endodontic sealers after 24 hr and 48 hr.
The sealers studied were Canason, Acroseal, Epiphany, AH-Plus and MTA, while the microorganisms used were Streptococcus viridans, Staphylococcus aureus and Enterococcus faecalis. Agar diffusion method on Muller Hinton agar was employed; fourty five petriplates with 25 ml of Muller Hinton agar were inoculated with 0.1 ml of the experimental suspension. Five cavities , each one measuring 5 ml in diameter and 4 ml in depth , were made in each agar plate using cork poorer and then completely filled with the product to be tested .
Canason containing eugenol and formaldehyde proved to be the most effective against all microorganisms tested. This was followed by Epiphany, Acroseal and AH-Plus which showed antibacterial activity on all tested microorganisms higher than that of MTA which showed the least action on all tested microorganisms. No antimicrobial activity was seen after 48 hrs.
All the sealers evaluated in this study showed different inhibitory effect against all bacterial