Effect Of Selective Dentin Etching And Ethanol Wet Bonding On Micro-Shear Bond Strength Of Adhesive

Authors

DOI:

https://doi.org/10.32828/mdj.v21i2.1206

Keywords:

Ethanol-wet bonding, Selective dentin etching, Micro-Shear bond strength, Scotchbond Universal plus

Abstract

Aim: This in vitro study aimed to evaluate the impact of selective dentin etching for 3 seconds and ethanol pre-treatment on the micro-shear bond strength (µSBS) of Scotchbond Plus Universal (SBU), a hydrophilic adhesive containing 2-hydroxyethyl methacrylate (HEMA).

Materials and Methods: Forty-eight sound premolars had their occlusal enamel ground to create flattened midcoronal dentin. Teeth were divided into four groups of 12 sample. In Group A (control), SBU plus was applied without prior treatment. Group B involved selective dentin etching with 37% phosphoric acid for 3 seconds. Group C treated dentin surfaces with 100% ethanol for 60 seconds. Group D combined 3-second phosphoric acid etching with 60-second ethanol pre-treatment. The adhesive resin was applied then light cured. Teeth were restored with Filtek Z350 (3M ESPE, USA) composite rods (1mm diameter). After composite placement, samples were stored in distilled water at 37°C for 24 hours and thermocycled for 500 cycles. µSBS was measured using a universal testing machine. Data were analyzed using one-way ANOVA.

Results: ANOVA revealed significant differences among the groups (P < 0.001). Group D (selective dentin etching + ethanol pre-treatment) showed the highest µSBS at 57.16 MPa. The number of mixed failures was higher when dentin surfaces were treated with selective etching for 3 seconds and 100% ethanol.

Conclusion: Under the conditions of this study, dentin etched with phosphoric acid for 3 seconds and rewetted with 100% 

References

1. Abdulrasool, Ahmed, and Abdulla Al-Shamma. "Effect of Chlorhexidine and/or Ethanol Pre-bonding Treatment on the Shear Bond Strength of Resin Composite to Dentin." International Journal of Medical Research & Health Sciences, vol. 8, no. 3, 2019, pp. 150-159. ISSN 2319-5886.

2. Al-Obaidi Z, Jasim H. Assessment of Shear Bond Strength to Sound and Artificial Caries Affected Dentin Using Different Adhesive Systems: An In Vitro Study. Dental Hypotheses. 2023;14:10.

3. Ahn J, Jung KH, Son SA, Hur B, Kwon YH, Park JK. Effect of additional etching and ethanol-wet bonding on the dentin bond strength of one-step self-etch adhesives. Restor Dent Endod. 2015;40(1):68-74. doi:10.5395/rde.2015.40.1.68.

4. Aggarwal V, Singla M, Sharma R, Miglani S, Bhasin SS. Effects of simplified ethanol-wet bonding technique on immediate bond strength with normal versus caries-affected dentin. J Conserv Dent. 2016;19(5):419-423. doi:10.4103/0972-0707.190009

5. Ayar MK. Ethanol application protocols and microtensile dentin bond strength of hydrophobic adhesive. Tanta Dent J. 2014;11(3):206–212.

6. Birlbauer S, Chiang ML, Schuldt C, et al. Shear bond strength and microleakage results for three experimental self-etching primer compositions for pit and fissure sealing. Clin Oral Investig. 2017;21(5):1465-1473. doi:10.1007/s00784-016-1907-z

7. Breschi, L., Mazzoni, A., and S. Nunes. "The Role of Matrix Metalloproteinases in the Degradation of Dentin Bonding Agents." Journal of Dentistry, vol. 56, no. 6, 2018, pp. 119-128.

8. Chen C, Niu LN, Xie H, et al. Bonding of universal adhesives to dentine--Old wine in new bottles?. J Dent. 2015;43(5):525-536. doi:10.1016/j.jdent.2015.03.004

9. Comba A, Baldi A, Pucci R, Rolando C, Alovisi M, Pasqualini D, Scotti N. Effects of Etching Time and Ethanol Wet Bonding on Bond Strength and Metalloproteinase Activity in Radicular Dentin. Journal of Clinical Medicine. 2024; 13(9):2474. https://doi.org/10.3390/jcm13092474.

10. De Munck J, Mine A, Van den Steen PE, et al. Enzymatic degradation of adhesive-dentin interfaces produced by mild self-etch adhesives. Eur J Oral Sci. 2010;118(5):494-501. doi:10.1111/j.1600-0722.2010.00758.x.

11. Deng D, Yang H, Guo J, Chen X, Zhang W, Huang C. Effects of different artificial ageing methods on the degradation of adhesive-dentine interfaces. J Dent. 2014;42(12):1577-1585. doi:10.1016/j.jdent.2014.09.010.

12. Ekambaram M, Yiu CK, Matinlinna JP, King NM, Tay FR. Adjunctive application of chlorhexidine and ethanol-wet bonding on durability of bonds to sound and caries-affected dentine. J Dent. 2014;42(6):709-719. doi:10.1016/j.jdent.2014.04.001.

13. Fadhil Al-Abd Al-Abbas, Mohammed & Al-Shamma, Abdulla.. Hybrid Layer Thickness and Resin Tags Penetration of two Universal Adhesives Incorporated with Ascorbic Acid Coated Superparamagnetic Nanoparticles Subjected to External Magnetic Field. International Journal for Pharmaceutical Research Scholars.(2020) (0975-2366) Volume 12. 1548-1555. doi:10.31838/ijpr/2020.SP1.238.

14. Gajjela RS, Satish RK, Sajjan GS, Varma KM, Rambabu T, Vijaya Lakshmi BH. Comparative evaluation of chlorhexidine, grape seed extract, riboflavin/chitosan modification on microtensile bond strength of composite resin to dentin after polymerase chain reaction thermocycling: An in vitro study. J Conserv Dent. 2017;20(2):120-124. doi:10.4103/0972-0707.212241.

15. Goldberg M, Kulkarni AB, Young M, Boskey A. Dentin: structure, composition and mineralization. Front Biosci (Elite Ed). 2011;3(2):711-735. Published 2011 Jan 1. doi:10.2741/e281

16. Goldberg M, Kulkarni AB, Young M, Boskey A. Dentin: structure, composition and mineralization. Front Biosci (Elite Ed). 2011;3(2):711-735. Published 2011 Jan 1. doi:10.2741/e281

17. Gönülol N, Ertaş E, Yılmaz A, Çankaya S. Effect of thermal aging on microleakage of current flowable composite resins. J Dent Sci. 2015; 10(4): 376–82.

18. Guimarães LA, Almeida JC, Wang L, D'Alpino PH, Garcia FC. Effectiveness of immediate bonding of etch-and-rinse adhesives to simplified ethanol-saturated dentin. Braz Oral Res. 2012;26(2):177-182. doi:10.1590/s1806-83242012000200015

19. Hameedi ZJ, Gholam MK. Evaluation of the shear bond strength of three different universal bonding agents using different etching techniques. AIP Conf Proc. 2023;2839.

20. Hao, L., Yanhui Liu, Jie Yang, Lei Wu, and Zhongzheng Zhang. "Ultrastructure of Dentin-Resin Interfaces after Different Bonding Treatments: A FESEM Study." Clinical Oral Investigations, vol. 26, no. 9, 2022, pp. 5393-5403.

21. Hardan L, Bourgi R, Kharouf N, et al. Bond Strength of Universal Adhesives to Dentin: A Systematic Review and Meta-Analysis. Polymers (Basel). 2021;13(5):814. Published 2021 Mar 7. doi:10.3390/polym13050814.

22. Hardan L, Orsini G, Bourgi R, et al. Effect of Active Bonding Application after Selective Dentin Etching on the Immediate and Long-Term Bond Strength of Two Universal Adhesives to Dentin. Polymers (Basel). 2022;14(6):1129. Published 2022 Mar 11. doi:10.3390/polym14061129.

23. Hattar S, Hatamleh MM, Sawair F, Al-Rabab'ah M. Bond strength of self-adhesive resin cements to tooth structure. Saudi Dent J. 2015;27(2):70-74. doi:10.1016/j.sdentj.2014.11.006

24. Irmak Ö, Baltacıoğlu İH, Ulusoy N, Bağış YH. Solvent type influences bond strength to air or blot-dried dentin. BMC Oral Health. 2016;16(1):77. Published 2016 Aug 22. doi:10.1186/s12903-016-0247-3.

25. Jessop N. Notched edge shear bond strength test. International Standard 2013. ISO 29022.

26. Kharouf N, Rapp G, Mancino D, Hemmerlé J, Haikel Y, Reitzer F. Effect of etching the coronal dentin with the rubbing technique on the microtensile bond strength of a universal adhesive system. Dent Med Probl. 2019;56(4):343-348. doi:10.17219/dmp/111697.

27. Khoroushi M, Rafiei E. Effect of thermocycling and water storage on bond longevity of two self-etch adhesives. Gen Dent. 2013;61(3):39-44.

28. Kokubo, Y., and A. Sadr. "Advancements in Mitigating Ester Bond Degradation in Dental Methacrylate Resins." Frontiers in Dental Medicine, vol. 5, 2024, article 923780, https://doi.org/10.3389/fdmed.2022.923780.

29. Li B, Zhu X, Ma L, et al. Selective demineralisation of dentine extrafibrillar minerals-A potential method to eliminate water-wet bonding in the etch-and-rinse technique. J Dent. 2016;52:55-62. doi:10.1016/j.jdent.2016.07.008

30. Li F, Liu XY, Zhang L, Kang JJ, Chen JH. Ethanol-wet bonding technique may enhance the bonding performance of contemporary etch-and-rinse dental adhesives. J Adhes Dent. 2012;14(2):113-120. doi:10.3290/j.jad.a21853

31. Liu Y, Tjäderhane L, Breschi L, et al. Limitations in bonding to dentin and experimental strategies to prevent bond degradation. J Dent Res. 2011;90(8):953-968. doi:10.1177/0022034510391799

32. Manfroi FB, Marcondes ML, Somacal DC, Borges GA, Júnior LH, Spohr AM. Bond Strength of a Novel One Bottle Multi-mode Adhesive to Human Dentin After Six Months of Storage. Open Dent J. 2016;10:268-277. Published 2016 Jun 6. doi:10.2174/1874210601610010268

33. Manihani AKDS, Mulay S, Beri L, Tandale A, Bhawalkar A, Dalsania R. Comparative evaluation of the effect of two natural collagen cross-linkers on microtensile bond strength of self-etch adhesive system to dentin after contamination with blood and hemostatic agent: An in vitro study. J Conserv Dent Endod. 2023;26(4):466-471. doi:10.4103/jcd.jcd_312_23.

34. Pashley DH, Tay FR, Breschi L, et al. State of the art etch-and-rinse adhesives. Dent Mater. 2011;27(1):1-16. doi:10.1016/j.dental.2010.10.016.

35. Pei D, Huang X, Huang C, Wang Y, Ouyang X, Zhang J. Ethanol-wet bonding may improve root dentine bonding performance of hydrophobic adhesive. J Dent. 2012;40(5):433-441. doi:10.1016/j.jdent.2012.02.005.

36. Porto ICCM, Nascimento TG, Oliveira JMS, Freitas PH, Haimeur A, França R. Use of polyphenols as a strategy to prevent bond degradation in the dentin-resin interface. Eur J Oral Sci. 2018;126(2):146-158. doi:10.1111/eos.12403

37. Sabri AM, Mahdee AF. The Effect of 45S5 Bioactive Glass Suspension Compared with Chlorhexidine on Early and Delayed Micro Shear Bond Strength to Sound versus Partially Demineralized Dentin. [M.Sc. thesis]. University of Baghdad, College of Dentistry. 2024.

38. Sadek FT, Castellan CS, Braga RR, et al. One-year stability of resin-dentin bonds created with a hydrophobic ethanol-wet bonding technique. Dent Mater. 2010;26(4):380-386. doi:10.1016/j.dental.2009.12.009

39. Salah Z, Sleibi A. Effect of deep margin elevation on fracture resistance of premolars restored with ceramic onlay: In vitro comparative study. J Clin Exp Dent. 2023;15(6):e446-e453. Published 2023 Jun 1. doi:10.4317/jced.60384.

40. Sartori N, Peruchi LD, Phark JH, et al. Permeation of intrinsic water into ethanol- and water-saturated, monomer-infiltrated dentin bond interfaces. Dent Mater. 2015;31(11):1385-1395. doi:10.1016/j.dental.2015.08.159

41. Saleh SA. Immediate and delayed micro shear bond strength evaluation of two glass ionomer cements to composite resin by using different bonding techniques—an in vitro study. BDJ Open. 2024;10. doi: 10.1038/s41405-024-00283-8.

42. Santos, Alessandra J., Gabriel S. de Mello, Márcia A. Chinelatti, and Angelo M. Carvalho. "Long-Term Bond Stability of Self-Etch Adhesives: A FESEM Evaluation of Resin Tags and Lateral Branches in Dentin." Journal of Adhesive Science and Technology, vol. 37, no. 5, 2023, pp. 984-995.

43. Sauro S, Watson TF, Mannocci F, et al. Two-photon laser confocal microscopy of micropermeability of resin-dentin bonds made with water or ethanol wet bonding. J Biomed Mater Res B Appl Biomater. 2009;90(1):327-337. doi:10.1002/jbm.b.31290

44. Simões DM, Basting RT, Amaral FL, Turssi CP, França FM. Influence of chlorhexidine and/or ethanol treatment on bond strength of an etch-and-rinse adhesive to dentin: an in vitro and in situ study. Oper Dent. 2014;39(1):64-71. doi:10.2341/12-486-L.

45. Spencer, P., Wang, Y., & Tamerler, C. "A review of the hybrid layer in adhesive dentistry." Journal of Adhesive Dentistry, 2020.22(3), 257-265.

46. Stape THS, Wik P, Mutluay MM, Al-Ani AAS, Tezvergil-Mutluay A. Selective dentin etching: A potential method to improve bonding effectiveness of universal adhesives. J Mech Behav Biomed Mater. 2018;86:14-22. doi:10.1016/j.jmbbm.2018.06.015.

47. Tay FR, Hashimoto M, Pashley DH, et al. Aging affects two modes of nanoleakage expression in bonded dentin. J Dent Res. 2003;82(7):537-541. doi:10.1177/154405910308200710.

48. Telles CC, Basting RT, Bridi EC, França FM, do Amaral FL, Basting RT. Wet-bonding technique with ethanol may reduce protease activity in dentin-resin interface following application of universal adhesive system. J Clin Exp Dent. 2023;15(5):e403-e410. Published 2023 May 1. doi:10.4317/jced.60307.

49. Van Meerbeek B, Yoshihara K, Van Landuyt K, Yoshida Y, Peumans M. From Buonocore's Pioneering Acid-Etch Technique to Self-Adhering Restoratives. A Status Perspective of Rapidly Advancing Dental Adhesive Technology. J Adhes Dent. 2020;22(1):7-34. doi:10.3290/j.jad.a43994

50. Van Meerbeek B, Yoshihara K, Yoshida Y, Mine A, De Munck J, Van Landuyt KL. State of the art of self-etch adhesives. Dent Mater. 2011;27(1):17-28. doi:10.1016/j.dental.2010.10.023.

51. Van Meerbeek, B., De Munck, J., & Vargas, M. State of the art of dentin bonding. Journal of Adhesive Dentistry, (2020). 22(6), 421-444. https://doi.org/10.3290/j.jad.a44225

52. You X, Chen L, Xu J, Li S, Zhang Z, Guo L. Effects of carbodiimide combined with ethanol-wet bonding pretreatment on dentin bonding properties: an in vitro study. PeerJ. 2022;10:e14238.2022 Oct 21. doi:10.7717/peerj.14238.

53. Venigalla BS, Jyothi P, Kamishetty S, Reddy S, Cherukupalli RC, Reddy DA. Resin bond strength to water versus ethanol-saturated human dentin pretreated with three different cross-linking agents. J Conserv Dent. 2016;19(6):555-559. doi:10.4103/0972-0707.194019

54. Voinot, J., Bedez, M. Pretreatments to bonding on enamel and dentin disorders: a systematic review. Evid Based Dent 25, 215(2024). https://doi.org/10.1038/s41432-024-01037-z

55. Walter R, Feiring AE, Boushell LW, et al. One-year water sorption and solubility of "all-in-one" adhesives. Braz Dent J. 2013;24(4):344-348. doi:10.1590/0103-6440201302267

56. Wang, X., Li, H., Chen, L., Wang, Y., Bai, J., Wang, D., & Liu, H. Effects of simplified ethanol–wet bonding and hydrophobic coating on resin–dentin bonding properties. Journal of Adhesion Science and Technology, (2020).35(9),913–927.

57. Yang H, Guo J, Deng D, Chen Z, Huang C. Effect of adjunctive application of epigallocatechin-3-gallate and ethanol-wet bonding on adhesive-dentin bonds. J Dent.2016;44:44-49. doi:10.1016/j.jdent.2015.12.001.

58. Yesilyurt C, Ayar MK, Yildirim T, Akdag MS. Effect of simplified ethanol-wet bonding on dentin bonding durability of etchand-rinse adhesives. Dent Mater J. 2015;34(4):441-448. doi:10.4012/dmj.2014-335.

59. Yoshihara K, Yoshida Y, Nagaoka N, et al. Nano-controlled molecular interaction at adhesive interfaces for hard tissue reconstruction. Acta Biomater. 2010;6(9):3573-3582. doi:10.1016/j.actbio.2010.03.024

60. Yi L, Yu J, Han L, Li T, Yang H, Huang C. Combination of baicalein and ethanol-wet-bonding improves dentin bonding durability. J Dent. 2019;90:103207. doi:10.1016/j.jdent.2019.103207.

61. Zhang Z, Wang X, Zhang L, et al. The contribution of chemical bonding to the short- and long-term enamel bond strengths. Dent Mater. 2013;29(7):e103-e112. doi:10.1016/j.dental.2013.04.009.

Downloads

Published

30.12.2025

How to Cite

Effect Of Selective Dentin Etching And Ethanol Wet Bonding On Micro-Shear Bond Strength Of Adhesive. (2025). Mustansiria Dental Journal, 21(2), 294-317. https://doi.org/10.32828/mdj.v21i2.1206

Similar Articles

71-80 of 168

You may also start an advanced similarity search for this article.