Anticandedal Effect action of Different Concentrations of Aglycon fraction of Anthroquinon (monoanathron) isolated from Aloe vera on Heat cure Acrylic Resins
DOI:
https://doi.org/10.32828/mdj.v16i1.1027Keywords:
Aloe Vera, denture stomatitis, aglycon, monoanthron, anthroquinonAbstract
Aim of study: to investigate the effects of different concentrations of aloe vera , aglycon part (monoanathron ) against candida albicance adhere to two types of heat cure acrylic resin denture base (conventional and Hi- impact) on two periods of time (30 minutes and 8 hours).
Materials & methods: A total of 120 specimens were prepared, sixty specimens of each hot cure denture base acrylic resin material, each sixty specimens were farther divided into two subgroups of 30 specimens according to the time of immersion, each 30 specimens were farther subdivided into three 10 specimen groups according to the concentration of aleo vera used, isolation of free aglycon (Monoianthron) from anthroquinon glycoside of aloe vera plant; Candida albicans isolation and identification and preparation of candida albicans suspension; autoclaved specimens of each sub-group for both heat cure acrylic resins were placed into the tubes containing brain heart infusion plus inoculum and remained for 12 hours at 37 0C in order to favor an initial colonization of the acrylic resin surfaces, the disinfection step was performed over two periods, colony formation was then counted after incubation.
Results: The two concentration of aloe Vera studied in the present investigation exhibited varying degree of inhibitory effect against candida albicans adherent to different type of heat cure denture base acrylic resins. The degree of inhibition varied depending on the concentration of the extract while the period of immersion has no significant effect on the number colony.
Conclusion: with the limitation of this study it can be concluded that 100% and 75% concentrations of monoanthron aglycon part of anthroquinon isolated from aloe vera have anticandidal effect, 100%concentration has higher effect than75%. Immertion period has no effect on the anticandidal activity of the solutions.
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