Anticandedal Effect action of Different Concentrations of Aglycon fraction of Anthroquinon (monoanathron) isolated from Aloe vera on Heat cure Acrylic Resins


  • Intisar Kadhum Farhood
  • Huda Saran B. Pharm
  • Farah Nabeel
  • Hanan Al-ansary



Aloe Vera, denture stomatitis, aglycon, monoanthron, anthroquinon


Aim of study: to investigate the effects of   different concentrations of aloe vera , aglycon part (monoanathron ) against candida albicance adhere   to two types of heat cure acrylic  resin denture base (conventional and Hi- impact) on two periods of time (30 minutes and 8 hours).

Materials & methods: A total of 120 specimens were prepared, sixty specimens of each hot cure denture base acrylic resin material, each sixty specimens were farther divided into two subgroups of 30 specimens according to the time of immersion, each 30 specimens were farther subdivided into three 10 specimen groups according to the concentration of aleo vera used, isolation of free aglycon (Monoianthron) from anthroquinon glycoside of aloe vera plant; Candida albicans isolation and identification and preparation of candida albicans suspension; autoclaved specimens of each sub-group for both heat cure acrylic resins were placed into the tubes containing brain heart infusion plus inoculum and remained for 12 hours at 37 0C in order to favor an initial colonization of the acrylic resin surfaces, the disinfection step was performed over two periods, colony formation was then counted after incubation.

Results:  The two concentration of aloe Vera studied in the present investigation exhibited varying degree of inhibitory effect against candida albicans adherent to different type of  heat cure denture base acrylic resins. The degree of inhibition varied depending on the concentration of the extract while the period of immersion has no significant effect on the number colony.

Conclusion: with the limitation of this study it can be concluded that 100% and 75% concentrations of monoanthron aglycon part of anthroquinon isolated from aloe vera have anticandidal effect, 100%concentration has  higher effect than75%. Immertion period has no effect on the anticandidal activity of the solutions.


- Bensadoun RJ, Patton LL, Lalla RV, Epstein JB. Oropharyngeal candidiasis in head and neck cancer patients treated with radiation:update 2011. Support Care Cancer. 2011; 19:737-44.

- Budtz-Jörgensen E, Stenderup A, Grabowski M. An epidemiologic study of yeasts in elderly denture wearers. Community Dent OralEpidemiol. 1975;3:115-9

- Marsh P, Martin M. Oral microbiology. 4th ed. Oxford. 1999: 153-6.

- Geerts GA, Stuhlinger ME, Basson NJ. Effect of an antifungal denture liner on the saliva yeast count in patients with denture stomatitis:a pilot study. J Oral Rehabil. 2008;35:664-9.

- M a c Farlane TW, Samaranayake LP. Fungal infections. In: Clinical Oral Microbiology. London: Wright, 1989:122-39.

- Dar-Odeh NS, Al-Beyari M, Abu-Hammad OA. The role of antifungal drugs in the management of denture – associated stomatitis. The International Journal of Antimicrobial Agents 2012; 2(1):1-5.

- Pires FR, Santos EB, Bonan PR, de Almeida OP, Lopes MA. Denture stomatitis and salivary Candida in Brazilian edentulous patients. J Oral Rehabil 2002;29:1115-9.

- Webb BC, Thomas CJ, Willcox MD, Harty DW, Knox KW. Candida-associated denture stomatitis. Aetiology and management: a review. Part 3. Treatment of oral candidosis. Aust Dent J 1998;43:244-9.

- Ellepola, AN, Samaranayake, LP. Adjunctive use of chlorhexidine in oral candidoses: a review.Oral Disease 2001; 7(1):11-7

- Z. U. Khan, R. Chandy, and K. E. Metwali, “Candida albicans strain carriage in patients and nursing staff of an intensive care unit: a study of morphotypes and resistotypes,” Mycoses. 2003; 46( 11-12,): 479–86..

- Essawi T, Srour M.Screening of some Palestinian medicinal plants for antibacterial activity. J Ethanopharmacol.2000; 70 (3):343-9

- Casaroto AR, Lara VS. Phytomedicines for Candida-associated denture stomatitis. Fitoterapia 2010; 81(5): 323-8.

- Atherton P. Aloe vera revisited. Br J Phytother. 1998;4:76–83

- Femenia ES, Sanchez S, Simal and Rossello C. Com- positional Features of Polysaccharides from Aloe vera (Aloe barbadensis Miller) Plant Tissues,.Carbohydrate Polymers. 1999;39(2):109-1

- Das S, B. Mishra B, Gill K, Ashraf MS, Singh AK, Sinha M, Sharma S, Xess I, Dalal K, Singh TP, Dey S. Isolation and characterization of novel protein with anti-fungal and anti-inflammatory properties from Aloe vera leaf gel. International. Journal of Biological Macromolecules. 2011; 48(1):38-43.

- Surjushe A, Vasani R, Saple DG. Aloe vera: a short review. Indian J Dermatol. 2008; 53(4):163-6.

- Nevzatoglu EU, Ozcan M, Kulak-Ozkan Y, Kadir T. Adherence of candida albicans to denture base acrylics and Silicon –based resilient liner materials with different surface finish. Clin Oral Invest. 1997;11:231-6.

- Cahn LR. The denture sore mouth. Ann Dent 1936;3:33-6.

- Salerno C, Pascale M, Contaldo M, Esposito V, Busciolano M, Milillo L, et al. Candida-associated denture stomatitis. Med Oral Patol Oral Cir Bucal 2011; 16 (2):e139-43.

- Casaroto AR, Lara VS. Phytomedicines for Candida-associated denture stomatitis. Fitoterapia 2010; 81(5): 323-8.

- Budtz-Jorgensen E. Sequehae caused by wearing complete dentures. In Zarb GA, Bolender CL (Eds). Prosthodontic treatment for edentulous patients: Complete dentures and Implant supported prosthesis (12th ed), Elsevier: New Delhi 2007; 34-50

- Anusavice KJ. Phillips. Science of Dental Materials. 11th ed. China: 2007

- van der Watt E, Pretorius JC. Purification and identification of active antibacterial components in Carpobrotus edulis L. J Ethnopharmacol 2001; 76:87-91.

- Chatterjee A, Saluja M, Singh N, Kandwal A. To evaluate the anti-gingivitis and antipalque effect of an Azadirachta indica (neem) mouthrinse on plaque induced gingivitis: A double-blind, ran¬domized, controlled trial. J Indian Soc Periodontol. 2011;15 (4):398– 401.

- Mahesh B, Satish S. Antimicrobial activity of some important medicinal plant against plant and human pathogens. World J. Agri.Sci. 2008; 4(5) : 839-43

- Ogundipe O, Akinbiyi O, Moody JO. Antibacterial activities of essential ornamental plants. Nigeria J. Natural Products & Medicine. 1998; 2: 46-7

- Bibith B, Jish VK, Salitha CV, Mohan S, Valsa AK, Antibacterial activity of different plant extracts Short communication. Indian J Microbial. 2002; 42:361-3

- Kassab-Bashi T. Abdul–Rahmann GH. kassab NH. Antifungal Effect of Some Medicinal Plant Ex-tracts On Candida Albicans Adherence on Acrylic Resin Denture Base Material. An In Vitro Study. Al–Rafidain Dent J. 2014; 14(1): 139-144.

- Marcos-Arias C, Eraso E, Madariagaet L, Quindós G: In vitro activities of natural products against oral Candida isolates from denture wearers. BMC Complementary and Alternative Medicine 2011; 11:119.

- Thaweboon S, Thaweboon B. Inhibition of candida adhesion to denture acrylic by Boesenbergia pandurata. Asian Pac J Trop Med. 2010:272-5.

- Sookto T, Srithavaj T, Thaweboon S, Thaweboon B, Shrestha B. In vitro effects of Salvia officinalis L. essential oil on Candida albicans. Asian Pac J Trop Biomed 2013; 3(5):376-80.

- Amanlou M, Beitollahi JM, Abdollahzadeh S, Tohidast-Ekrad Z. Miconazole gel compared with Zataria multiflora Boiss. gel in the treatment of denture stomatitis. Phytother Res 2006; 20:966-9.

- Sabzghabaee AM, Davoodi N, Ebadian B, Aslani A, Ghannadi A. Clinical evaluation of the essential oil of Satureja hortensis for the treatment of denture stomatitis. Dent Res J 2012; 9(2):198-202.

- Sabzghabaee AM, Shirdare Z, Ebadian B, Aslani A, Ghannadi A. Clinical evaluation of the essential oil of Pelargonium graveolens for the treatment of denture stomatitis. Dent Res J 2011; 8 (1):105–8.

- Taweechaisupapong S, Klanrit P, Singhara S, Pitiphat W, Wongkham S. Inhibitory effect of Streblus asper leaf-extract on adhesion of Candida albicans to denture acrylic. J Ethnopharmacol 2006;106:414-7.

- Barani K, Manipal S, Prabu D, Ahmed A, Adusumilli P, Jeevika C. Anti-fungal activity of Morinda citrifolia (noni) extracts against Candida albicans: An in vitro study. Indian J Dent Res 2014;25:188-90.

- Surjushe A, Vasani R, Saple DG. Aloe vera: a short review. Indian J Dermatol. 2008; 53(4): 163–6.

- Johnson, M., Renisheya, J.M, Nancy, B.S., Laju, R.S., Aruriya, G. and Renola, J.T. Antimicrobial and Antifungal activity of Aloe vera Gel Extract. Journal of International Biomedical and advance research. 2012; 3:184-7.

- Oral ulcers remedy gets FDA clearance. The Journal of the American Dental Association, 1994; 125: 1308–10,

- Chandrahas B1, Jayakumar A, Naveen A, Butchibabu K, Reddy PK, Muralikrishna T. A randomized, doubleblind clinical study to assess the antiplaque and antigingivitis efficacy of Aloe vera mouth rinse. J Indian Soc Periodontol. 2012 Oct;16(4):543-8.

- Bhat G, Kudva P, Dodwad V. Aloe vera: Nature's soothing healer to periodontal disease. J Indian Soc Periodontol. 2011;15: 205–9.

- Hayes SM. Lichen planus—Report of successful treatment with aloe vera. Gen Dent 1999;47(3):268-72.

- Kathuria, N., Gupta, N., & Prasad, R. Biologic Effects Of Aloe Vera Gel. The Internet Journal of Microbiology. 2011; 9(2)

- Tello CG, Ford P, Iacopino AM. In vitro evaluation of complex carbohydrate denture adhesive formulations. Quintessence Int 1998;29(9):585-93.

- Nebedum J, Ajeigbe K, Nwobodo E, Uba C, Adesanya O, Fadare O, Ofusori D.2009. Comparative study of the ethanolic extracts of foure Nigerian plants against some pathogenic microorganisms, Res. J. Med. Plant. 2009; 3:23-8.

- Rosca-Casian O, Parvu M, Vlase L, Tamas M. 2007. Antifungal activity of Aleo vera leaves. Fitotherapia. 2007;78:219-22

- Subramanian S, Kumar DS, Arulselvain P, Senthilkumar, 2006. In vitro antibacterial and antifungal activities of ethanolic extract of aleo vera leaf gel. J Plant. Sci.2006;1: 348-55.

- Shamm S, Ahmed SW, Azhar I. Antifungal activity of Allum, Aleo and Solanum species. Pharm. Biol.2004; 42: 491-8

- Ali MIA., Shalaby NMM., Elgamal MHA., Mosa ASM. Antifungal effects of different plant extracts and their major components of selected Aloe species. Phytother.Res. 1999; 13:401-7

- Saka Y., Barkai-Golan R, 1995. Aleo vera activity against plant pathogen fangi. Postharvest. Biol. Tech. 1995; 6: 159-65

- Nidiry E S J, Ganeshan G, Lokesha AN.Antifungal Activity of Some Extractives and Constituents of Aleo vera. Res. J.Med. Plant. 2011;5(2): 196-200.

- George D, Bhat SS, Antony B. Comparative evaluation of the antimicrobial efficacy of Aloe vera tooth gel and two popular commercial toothpastes: An in vitro study. Gen Dent 2009;57:238-41.

- Shireen F, Manipal S, Prabu D. Anti-fungal activity of Aloe vera: In vitro study. SRM J Res Dent Sci 2015;6:92-5.

- Saran H, Rafeeq AK, Abd- Alhaneed SM, Kadhum I. Antifungal Evaluation of Different Concentrations of Aglycon fraction of Anthroquinon (Dianthron) isolated from Aleo Vera on Acrylic Resins. Proceedings of The First International Conference for the University College for Humanitarian Studies, 2016; 1st part 145-61

- Heggers JP, Pineless GR, Robson MC. Dermaide aloe/Aloe vera gel: Comparison of the antimicrobial effects. J Am Med Technol. 1979;41:293-4.

- Joseph B, Raj SJ, Pharmacognostic and phytochemical properties of Aloe vera linn –an overview, International Journal of Pharmaceutical Sciences Review and Research. 2010;4(2): 106-10.

- L. Lorenzetti, R. Salisbury, J. Beal, J. Baldwin, “Bacteriostatic Property of Aloe vera”, J. Pharmacol. Sci. 1964; 3: 1287

- Budtz-Jorgensen E, Carlino P. A miconazole lacquer in the treatment of Candida-associated denture stomatitis. Mycoses 1994; 37:131-5.

- Pesci-Bardon C, Fosse T, Madinier L, Serre D. In vitro new dialysis protocol to assay the antisepticproperties of a quaternary ammonium compound polymerized with denture acrylic resin. 2004 The Society for Applied Microbiology, Letters in Applied Microbiology. 2004; 39: 226–31

- Rathore P, Hegde A, Ginjupalli K, Upadhya PN. Evaluation of Antifungal Activity of Additives to Resilient Liners: An in vitro Pilot Study. 6Trends Biomater. Artif. Organs.2009; 23(1):6-9

- Nikawa H, Yamamoto T, Hamada T, Rahardjo MB, Murata H, Nakanoda S. Antifungal effect of zeolite-incorporated tissue conditioner against Candida albicans growth and/or acid production. J Oral Rehabil 1997;24:350-357.

- Addy M. In vitro studies into the use of denture base and soft liner materials as carriers for drugs in the mouth. JOral Rehabil 1981;8:131-142.

- Addy M, Handley R. The effects of the incorporation of chlorhexidine acetate on some physical properties of polymerized and plasticized acrylics. J OralRehabil1981;8:155-163.




How to Cite

Kadhum Farhood, I. ., Saran B. Pharm, H. ., Nabeel, F. ., & Al-ansary, H. . (2024). Anticandedal Effect action of Different Concentrations of Aglycon fraction of Anthroquinon (monoanathron) isolated from Aloe vera on Heat cure Acrylic Resins. Mustansiria Dental Journal, 16(1), 77–88.