@article{Mahmood_Ali_Abdul-Kadher_2019, title={The role of intracanal medicaments in inhibition of bacteria isolated from root canals of infected primary molars}, volume={14}, url={https://mdj.uomustansiriyah.edu.iq/index.php/mdj/article/view/766}, DOI={10.32828/mdj.v14i1.766}, abstractNote={<p>Microbes are considered as the primary etiologic agents in endodontic diseases.<br>Disinfection of the root canal is obtained by the combined effect of biomechanical<br>preparation, irrigation and intra canal medicament. The aim of the present study was<br>to assess the antimicrobial activity of intracanal medicaments (formocresol and<br>Endosepton) against two micro organisms (Streptococcus mutans and staphylococcus<br>aureus) isolated from 15 necrotic pulps of primary molars indicated for pulpectomy<br>procedure. The samples were cultured, and purified using microbiological evaluation.<br>Broth dilution test was performed in our study by preparing test tubes containing<br>10 ml of BHI broth (pH. 7) which then inoculated with strains of the tested bacteria<br>and incubated at 37 C° for 24 h. After over night incubaction, ten fold dilution were<br>made in test tubes containing 9 ml of normal saline by adding 1 ml of the inoculum to<br>the first tube . Then from dilution 10-1 , 0.1 ml of cell suspension was added to 9.9 ml<br>of formocresol and endosepton, then 0.1 ml was taken and spread on duplicates of<br>BHI agar plates at different intervals and incubated aerobically for 24 h. at 37 C°.<br>Colonies on the plates were counted after incubation and CFU/mL (colony forming<br>unit) was calculated. Our results indicating that there were no significant differences<br>between the intracanal medicaments, but there were high significant differences<br>between the intervals time of the study. We concluded that both materials had great<br>antibacterial effect against the pathogens commonly isolated from necrotic pulpal<br>tissue of primary teeth.<br><br></p>}, number={1}, journal={Mustansiria Dental Journal}, author={Mahmood, Dr. Maha Abdul- Kareem and Ali, Dr. Huda Elias and Abdul-Kadher, Dr. Haraa Khairi}, year={2019}, month={Jan.}, pages={92–98} }